|First appearance||"The Manotaur"|
|Age||Approximately 30 - 40|
|Residence||The Manotaur's house|
|Species||Half-human (50%), Half-horse (50%) (Minotaur)|
The Manotaur is a half-man with hairy hooves but all man (if one counts collecting comic books and action figures manly). He is a large man with a bald head who dresses like a city man. His plan was to kidnap all the Twonicorns and to shave off all their hair to make a large wig to cover up his really smooth, bald head to get a date with the Womanotaur. When Yin and Yang reach his lair, which is really his house in a cul-de-sac, Yang uses Man Smash, learned from his comicbook hero, U-Dude, and frees the Twonicorns. He returns in Game Over where he attempts to confiscate the Dojo for not paying bills, which he would "tear down and build an overpriced condo" only to fail thanks to Dave. The Manotaur is explicitly noted for having five fingers on each hand, as opposed to the "normal" four on everyone else in the show. He apparently adores lawyers due to their high pay.
- The Manotaur's voice actor, Seth MacFarlane, who is best known for creating and starring in Family Guy and American Dad, is one of the only three American voice actors to voice a fictional character in the series, alongside Orlando Brown (of That's So Raven), who voices Badfoot and James Belushi voiced The Puffin. Mitchell Musso and Jason Earles, two American teen actors, also guest starred in the show but played fictionalized versions of themselves and television personality Nancy O'Dell plays a parody of herself, Nancy O. Delffa.
- The manotaur lookes like a centaur, but a centaur has 4 legs and 2 arms and a minotaur has 2 legs and 2 arms.